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| Gerard, Andre; Naciri, Yamama; Noiret, Christophe; Ledu, Christophe; Peignon, Jean-marie. |
| For the first time, effective treatments using cytochalasin B were developed to induce triploidy in the European clam, Ruditapes decussatus (L.). The percentage of triploid embrios was assessed by karyological or image analysis. Two treatments (O.5 or 1 mg of cytochasin B (CB per ml of dimethylsulfoxide in 1 l of sea water) were applied at different times after fertilization (10 to 25 mn), for two different periods (15 and 20 min). Best results were obtained for a CB concentration of 1 mg/l. When treatment was applied 15 min after fertilization for a 20 min period, 94% and 95% of triploid embryos were obtained in two repeated experiments. At metamorphosis, the treated larvae appeared to be no smaller than the control larvae in all experiments. Thowever, in... |
| Tipo: Text |
Palavras-chave: Genetic; Ruditapes decussatus; Clam; Induced triploidy. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/1994/publication-3208.pdf |
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